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Addgene

pLV.PGK.GFP.miR9T
(Plasmid #115973)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115973 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLV
  • Backbone size w/o insert (bp) 6475
  • Total vector size (bp) 7897
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFPmiR9T
  • Species
    Synthetic
  • Insert Size (bp)
    1422
  • Promoter PGK
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer cctgtgttgccacctggatt
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV.PGK.GFP.miR9T was a gift from Johan Jakobsson (Addgene plasmid # 115973 ; http://n2t.net/addgene:115973 ; RRID:Addgene_115973)

  • For your References section:

    Visualization and genetic modification of resident brain microglia using lentiviral vectors regulated by microRNA-9. Akerblom M, Sachdeva R, Quintino L, Wettergren EE, Chapman KZ, Manfre G, Lindvall O, Lundberg C, Jakobsson J. Nat Commun. 2013;4:1770. doi: 10.1038/ncomms2801. 10.1038/ncomms2801 PubMed 23612311
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