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Addgene

N-WASP (WGP)
(Plasmid #11597)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11597 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    N-WASP
  • Alt name
    Neural Wiskott-Aldrich Syndrome Protein
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1300
  • Mutation
    contains amino acids 1-396
  • GenBank ID
    D88461
  • Entrez Gene
    Iqub (a.k.a. Trs4, MGC116166)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site FseI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    N-WASP (WGP) was a gift from Marc Kirschner (Addgene plasmid # 11597 ; http://n2t.net/addgene:11597 ; RRID:Addgene_11597)

  • For your References section:

    Mechanism of N-WASP activation by CDC42 and phosphatidylinositol 4, 5-bisphosphate. Rohatgi R, Ho HY, Kirschner MW. J Cell Biol. 2000 Sep 18. 150(6):1299-310. 10.1083/jcb.150.6.1299 PubMed 10995436
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