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Addgene

pTW072M
(Plasmid #115960)

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This material is available to academics and nonprofits only. Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
Item Catalog # Description Quantity Price (USD)
Plasmid 115960 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lvl2 (nsP2 Q739L)
  • Species
    venezuelan equine encephalitis
  • Mutation
    nsP2 Q739L

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTW072M was a gift from Ron Weiss (Addgene plasmid # 115960 ; http://n2t.net/addgene:115960 ; RRID:Addgene_115960)

  • For your References section:

    Small-molecule-based regulation of RNA-delivered circuits in mammalian cells. Wagner TE, Becraft JR, Bodner K, Teague B, Zhang X, Woo A, Porter E, Alburquerque B, Dobosh B, Andries O, Sanders NN, Beal J, Densmore D, Kitada T, Weiss R. Nat Chem Biol. 2018 Nov;14(11):1043-1050. doi: 10.1038/s41589-018-0146-9. Epub 2018 Oct 16. 10.1038/s41589-018-0146-9 PubMed 30327560
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