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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11594 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecdc20
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SpeciesH. sapiens (human)
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Insert Size (bp)1800
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GenBank IDNM_001255
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Entrez GeneCDC20 (a.k.a. CDC20A, OOMD14, OZEMA14, bA276H19.3, p55CDC)
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HA-cdc20 was a gift from Marc Kirschner (Addgene plasmid # 11594 ; http://n2t.net/addgene:11594 ; RRID:Addgene_11594) -
For your References section:
Substrate recognition by the Cdc20 and Cdh1 components of the anaphase-promoting complex. Pfleger CM, Lee E, Kirschner MW. Genes Dev. 2001 Sep 15. 15(18):2396-407. 10.1101/gad.918201 PubMed 11562349