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Addgene

MT-cdc20
(Plasmid #11593)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11593 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cdc20
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1800
  • GenBank ID
    NM_001255
  • Entrez Gene
    CDC20 (a.k.a. CDC20A, OOMD14, OZEMA14, bA276H19.3, p55CDC)
  • Tag / Fusion Protein
    • myc (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MT-cdc20 was a gift from Marc Kirschner (Addgene plasmid # 11593 ; http://n2t.net/addgene:11593 ; RRID:Addgene_11593)
  • For your References section:

    Substrate recognition by the Cdc20 and Cdh1 components of the anaphase-promoting complex. Pfleger CM, Lee E, Kirschner MW. Genes Dev. 2001 Sep 15. 15(18):2396-407. 10.1101/gad.918201 PubMed 11562349