pTW080
(Plasmid
#115928)
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PurposeSGP insert for replicon MoClo
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 115928 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLvl0 -98/30 SGP NA (No Aptamer)
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Speciesvenezuelan equine encephalitis
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTW080 was a gift from Ron Weiss (Addgene plasmid # 115928 ; http://n2t.net/addgene:115928 ; RRID:Addgene_115928) -
For your References section:
Small-molecule-based regulation of RNA-delivered circuits in mammalian cells. Wagner TE, Becraft JR, Bodner K, Teague B, Zhang X, Woo A, Porter E, Alburquerque B, Dobosh B, Andries O, Sanders NN, Beal J, Densmore D, Kitada T, Weiss R. Nat Chem Biol. 2018 Nov;14(11):1043-1050. doi: 10.1038/s41589-018-0146-9. Epub 2018 Oct 16. 10.1038/s41589-018-0146-9 PubMed 30327560