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PurposeExpression of dCas9 gene under the control of a weak Ptet promoter in the E. coli chromosome
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Depositing Lab
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Sequence Information
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Full plasmid sequence is not available for this item.
No maps are available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Bacterial Strain | 115925 | Bacteria in agar stab | 1 | $85 | |
Backbone
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Vector backbonenone
Growth in Bacteria
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Bacterial Resistance(s)None
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Growth Temperature37°C
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Growth Strain(s)E. coli K12 MG1655
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Copy numberUnknown
Gene/Insert
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Gene/Insert namenone
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
E. coli K12 MG1655 genotype: F- λ- ilvG- rfb-50 rph-1
Integration at lambda attB: pOSIP-KL-mCherry
Integration at primary 186 attB: pOSIP-CO-RBS-librarydCas9 (2-3)*
*Number in parentheses refers to the selected colony number.
mCherry quantifies dCas9 repression
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LC-E75 was a gift from David Bikard (Addgene plasmid # 115925) -
For your References section:
A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9. Cui L, Vigouroux A, Rousset F, Varet H, Khanna V, Bikard D. Nat Commun. 2018 May 15;9(1):1912. doi: 10.1038/s41467-018-04209-5. 10.1038/s41467-018-04209-5 [pii] PubMed 29765036