GFP-GW182delta1
(Plasmid #11592)

• Depositing Lab: Edward Chan
• Publication: Jakymiw et al Nat Cell Biol. 2005 Dec . 7(12):1267-74.

Backbone

• Vector backbone: custom-made pDEST:gfp vector
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GW182
• Species: H. sapiens (human)
• Insert Size (bp): 1500
• Mutation: contains only aa1-498; SEE DEPOSITOR NOTES BELOW
• GenBank ID: AY035864
• Entrez Gene: TNRC6A (a.k.a. CAGH26, FAME6, GW1, GW182, TNRC6)
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: StuI (destroyed during cloning)
• 5′ sequencing primer: GACCGCCGCCGGGATCACT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the plasmid contains amino acids 1-498 from NCBI reference AY035864. A longer isoform with an additional 253 amino acids on the N-terminus was subsequently discovered in 2008, and this plasmid contains amino acids 254-751 relative to the longer isoform. This plasmid will function as described in the associated publication.