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Addgene

pBS-f1(+)-ACTB-iTagRFP-T
(Plasmid #115915)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115915 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript
  • Backbone size w/o insert (bp) 2960
  • Total vector size (bp) 5870
  • Modifications to backbone
    added homology arms and a fluorescent protein.
  • Vector type
    donor template

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ACTB-TagRFP-T
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2700
  • Entrez Gene
    ACTB (a.k.a. BKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8)
  • Tag / Fusion Protein
    • TagRFP-T

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Insert is based on Addgene plasmid: AICSDP-15:ACTB-mEGFP (Plasmid #87425).

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

TagRFP-T was inserted. Please visit https://www.biorxiv.org/content/10.1101/864199v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-f1(+)-ACTB-iTagRFP-T was a gift from Erik Sontheimer (Addgene plasmid # 115915 ; http://n2t.net/addgene:115915 ; RRID:Addgene_115915)

  • For your References section:

    Efficient Homology-Directed Repair with Circular Single-Stranded DNA Donors. Iyer S, Mir A, Vega-Badillo J, Roscoe BP, Ibraheim R, Zhu LJ, Lee J, Liu P, Luk K, Mintzer E, Guo D, Soares de Brito J, Emerson CP Jr, Zamore PD, Sontheimer EJ, Wolfe SA. CRISPR J. 2022 Oct;5(5):685-701. doi: 10.1089/crispr.2022.0058. Epub 2022 Sep 7. 10.1089/crispr.2022.0058 PubMed 36070530
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