pcDNA_DENV2-NS2B3_V5
(Plasmid #115906)

• Purpose: Mammalian expression of DENV2 NS2B3 protein with a C-terminal V5 tag
• Depositing Lab: Alan Rothman
• Publication: Udawatte et al Front Cell Infect Microbiol. 2022 Oct 14;12:926036. doi: 10.3389/fcimb.2022.926036. eCollection 2022.

Backbone

• Vector backbone: pcDNA/V5-DEST
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 6700
• Total vector size (bp): 9000
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: DENV2 NS2B3 protease
• Species: Dengue Virus Serotype 2, Strain NGC
• Insert Size (bp): 2355
• GenBank ID: FJ390389.1
• Promoter: CMV
• Tag / Fusion Protein:
  • V5 tag (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7 primer
• 3′ sequencing primer: TK polyA

Resource Information

• Supplemental Documents:
  • DENV2 NS2B3 insert_primer walk.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry