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Addgene

pcDNA5-MIS12-LAP-BUB1
(Plasmid #115904)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115904 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MIS12-BUB1
  • Species
    H. sapiens (human)
  • Mutation
    BUB1 fused to MIS12
  • GenBank ID
    BUB1 Gene ID: 699
  • Entrez Gene
    MIS12 (a.k.a. 2510025F08Rik, KNTC2AP, MTW1, hMis12)
  • Tag / Fusion Protein
    • EYFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5-MIS12-LAP-BUB1 was a gift from Geert Kops (Addgene plasmid # 115904 ; http://n2t.net/addgene:115904 ; RRID:Addgene_115904)

  • For your References section:

    Dissecting the roles of human BUB1 in the spindle assembly checkpoint. Vleugel M, Hoek TA, Tromer E, Sliedrecht T, Groenewold V, Omerzu M, Kops GJ. J Cell Sci. 2015 Aug 15;128(16):2975-82. doi: 10.1242/jcs.169821. Epub 2015 Jul 6. 10.1242/jcs.169821 PubMed 26148513
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