GFP-hAgo2
(Plasmid #11590)

• Depositing Lab: Edward Chan
• Publication: Jakymiw et al Nat Cell Biol. 2005 Dec . 7(12):1267-74.

Backbone

• Vector backbone: custom-made pDEST:gfp vector
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Ago2
• Alt name: EIF2C2
• Species: H. sapiens (human)
• Insert Size (bp): 2600
• Entrez Gene: AGO2 (a.k.a. CASC7, EIF2C2, LESKRES, LINC00980, PPD, Q10)
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: GACCGCCGCCGGGATCACT

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Tom Hobman, University of Alberta, Edmonton, Canada
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry