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pcDNA5-LAP-KNL1-M3
(Plasmid #115896)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115896 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KNL1-M3
  • Species
    H. sapiens (human)
  • Mutation
    Fragment of KNL1 containing three KNL1 repeat sequences
  • Entrez Gene
    KNL1 (a.k.a. AF15Q14, CASC5, CT29, D40, MCPH4, PPP1R55, Spc7, hKNL-1, hSpc105)
  • Tag / Fusion Protein
    • EYFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

siRNA resistant

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5-LAP-KNL1-M3 was a gift from Geert Kops (Addgene plasmid # 115896 ; http://n2t.net/addgene:115896 ; RRID:Addgene_115896)

  • For your References section:

    Arrayed BUB recruitment modules in the kinetochore scaffold KNL1 promote accurate chromosome segregation. Vleugel M, Tromer E, Omerzu M, Groenewold V, Nijenhuis W, Snel B, Kops GJ. J Cell Biol. 2013 Dec 23;203(6):943-55. doi: 10.1083/jcb.201307016. 10.1083/jcb.201307016 PubMed 24344183
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