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Addgene

Lox-Stop-Lox TOPO
(Plasmid #11584)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11584 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR-Blunt II-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3500
  • Vector type
    Mammalian Expression, Cre/Lox
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    However, plasmid yield and stability is improved when grown at 30 degrees.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    loxP-STOP-loxP
  • Species
    SV40
  • Insert Size (bp)
    5700

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid containing LoxP-STOP-LoxP cassette for use in expressing genes only after cre-mediated recombination.

Please also see Lox-Stop-Lox TOPO Hygro (http://www.addgene.org/35688) for a Hygromycin version of this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lox-Stop-Lox TOPO was a gift from Tyler Jacks (Addgene plasmid # 11584 ; http://n2t.net/addgene:11584 ; RRID:Addgene_11584)

  • For your References section:

    Analysis of lung tumor initiation and progression using conditional expression of oncogenic K-ras. Jackson EL, Willis N, Mercer K, Bronson RT, Crowley D, Montoya R, Jacks T, Tuveson DA. Genes Dev. 2001 Dec 15. 15(24):3243-8. 10.1101/gad.943001 PubMed 11751630
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