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Purposelentiviral, trimethoprim (TMP) regulated YAP promoter activity reporter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 115798 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTRE
- Total vector size (bp) 6000
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Modifications to backboneEGFP promoter and EGFP cloned out
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Vector typeMammalian Expression, Lentiviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameYAP responsive promoter
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Alt name8xGTIIC
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SpeciesH. sapiens (human)
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Entrez GeneYAP1 (a.k.a. COB1, YAP, YAP-1, YAP2, YAP65, YKI)
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Tag
/ Fusion Protein
- DsRED-DHFR
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTRE-8XGTIIC-DsRED-DD was a gift from Joan Massague (Addgene plasmid # 115798 ; http://n2t.net/addgene:115798 ; RRID:Addgene_115798) -
For your References section:
Pericyte-like spreading by disseminated cancer cells activates YAP and MRTF for metastatic colonization. Er EE, Valiente M, Ganesh K, Zou Y, Agrawal S, Hu J, Griscom B, Rosenblum M, Boire A, Brogi E, Giancotti FG, Schachner M, Malladi S, Massague J. Nat Cell Biol. 2018 Aug;20(8):966-978. doi: 10.1038/s41556-018-0138-8. Epub 2018 Jul 23. 10.1038/s41556-018-0138-8 [pii] PubMed 30038252