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Addgene

pDC158
(Plasmid #115793)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115793 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET31b(+)
  • Backbone manufacturer
    Novagen
  • Total vector size (bp) 6014
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SNAP25
  • Species
    R. norvegicus (rat)
  • Mutation
    c84s, c85s, c90s, c92s
  • Entrez Gene
    Snap25 (a.k.a. SNAP-25B, SNAP-25a)
  • Promoter T7
  • Tag / Fusion Protein
    • hexa histidine (N terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Daniel Cipriano

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDC158 was a gift from Axel Brunger (Addgene plasmid # 115793 ; http://n2t.net/addgene:115793 ; RRID:Addgene_115793)

  • For your References section:

    Molecular Mechanisms of Synaptic Vesicle Priming by Munc13 and Munc18. Lai Y, Choi UB, Leitz J, Rhee HJ, Lee C, Altas B, Zhao M, Pfuetzner RA, Wang AL, Brose N, Rhee J, Brunger AT. Neuron. 2017 Aug 2;95(3):591-607.e10. doi: 10.1016/j.neuron.2017.07.004. 10.1016/j.neuron.2017.07.004 PubMed 28772123
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