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Addgene

pDC191
(Plasmid #115792)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115792 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET31b(+)
  • Backbone manufacturer
    Novagen
  • Total vector size (bp) 5731
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Synaptobrevin2
  • Species
    R. norvegicus (rat)
  • Mutation
    S28C and C103S (please see comments below)
  • Entrez Gene
    Vamp2 (a.k.a. RATVAMPB, RATVAMPIR, SYB, Syb2)
  • Promoter T7
  • Tag / Fusion Protein
    • hexa histidine (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

C103S is an additional mutation compared to NCBI reference- This will not affect plasmid function

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDC191 was a gift from Axel Brunger (Addgene plasmid # 115792 ; http://n2t.net/addgene:115792 ; RRID:Addgene_115792)

  • For your References section:

    Molecular Mechanisms of Synaptic Vesicle Priming by Munc13 and Munc18. Lai Y, Choi UB, Leitz J, Rhee HJ, Lee C, Altas B, Zhao M, Pfuetzner RA, Wang AL, Brose N, Rhee J, Brunger AT. Neuron. 2017 Aug 2;95(3):591-607.e10. doi: 10.1016/j.neuron.2017.07.004. 10.1016/j.neuron.2017.07.004 PubMed 28772123
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