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Addgene

Pinducer20-dCas9-SunTag-2A-BFP
(Plasmid #115779)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115779 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Pinducer 20
  • Backbone manufacturer
    Stephen Elledge
  • Backbone size w/o insert (bp) 11791
  • Total vector size (bp) 16319
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dCas9-SunTag-2A-BFP
  • Insert Size (bp)
    6000
  • Promoter TRE

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer GGGGACAAGTTTGTACAAAAAAGCAGGCTccGACAAGAAGTACAGCATCGGCCT
  • 3′ sequencing primer GGGGACCACTTTGTACAAGAAAGCTGGGTCatcgataagccctgcaggTTAGAC
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Pinducer20-dCas9-SunTag-2A-BFP was a gift from Margaret Goodell (Addgene plasmid # 115779 ; http://n2t.net/addgene:115779 ; RRID:Addgene_115779)

  • For your References section:

    Homeobox oncogene activation by pan-cancer DNA hypermethylation. Su J, Huang YH, Cui X, Wang X, Zhang X, Lei Y, Xu J, Lin X, Chen K, Lv J, Goodell MA, Li W. Genome Biol. 2018 Aug 10;19(1):108. doi: 10.1186/s13059-018-1492-3. 10.1186/s13059-018-1492-3 [pii] PubMed 30097071
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