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Addgene

pcDNA3.1-Tat101(AD8)-Flag
(Plasmid #115777)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115777 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(-)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HIV-1 Tat protein (AD8)
  • Mutation
    None
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Also see Khoury et al. Retrovirology (2018) 15:36
https://doi.org/10.1186/s12977-018-0421-6

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-Tat101(AD8)-Flag was a gift from Damian Purcell (Addgene plasmid # 115777 ; http://n2t.net/addgene:115777 ; RRID:Addgene_115777)

  • For your References section:

    High Throughput In Vitro Assessment of Latency Reversing Agents on HIV Transcription and Splicing. Khoury G, Purcell DFJ. J Vis Exp. 2019 Jan 22;(143). doi: 10.3791/58753. 10.3791/58753 PubMed 30735158
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