Skip to main content
Addgene

pCMV-Rev(NL4.3)
(Plasmid #115776)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115776 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pHIS
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HIV-1 Rev protein (NL4.3)
  • Mutation
    None
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Also see Khoury et al. Retrovirology (2018) 15:36
https://doi.org/10.1186/s12977-018-0421-6

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-Rev(NL4.3) was a gift from Damian Purcell (Addgene plasmid # 115776 ; http://n2t.net/addgene:115776 ; RRID:Addgene_115776)

  • For your References section:

    High Throughput In Vitro Assessment of Latency Reversing Agents on HIV Transcription and Splicing. Khoury G, Purcell DFJ. J Vis Exp. 2019 Jan 22;(143). doi: 10.3791/58753. 10.3791/58753 PubMed 30735158
Related items:
Commonly requested with: