pLTR.gp140/EGFP.Revdelta38/DsRed
(Plasmid #115775)

• Purpose: HIV splicing reporter allows the assessment of the effect of LRAs on HIV-1 transcription and splicing.
• Depositing Lab: Damian Purcell
• Publication: Khoury et al J Vis Exp. 2019 Jan 22;(143). doi: 10.3791/58753.

Backbone

• Vector backbone: pUC18
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Envelope fused to EGFP (gp140-EGFP) and non-functional Rev protein fused to DsRed fluorescent protein (Rev-delta38-DsRed).
• Mutation: gp140 uncleaved (1190-91aa, KR > TG; truncated prior to the transmembrane domain at amino acid 1363); Revdelta38
• Promoter: HIV-1 (LTR)
• Tag / Fusion Protein:
  • EGFP; dsReD (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • Splicing reporter EGFP-dsRed.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Also see Khoury et al. Retrovirology (2018) 15:36
https://doi.org/10.1186/s12977-018-0421-6