pCMV6-XL4 FLAG-SFRP2-Fc
(Plasmid #115772)

• Purpose: Expression of the extracellular domain of SFRP2 with N-terminal FLAG tag and C-terminal hIgG tag.
• Depositing Lab: Davide Comoletti
• Publication: Comoletti Lab Plasmids (unpublished)

Backbone

• Vector backbone: pCMV6-XL4
• Backbone size w/o insert (bp): 111
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SFRP2
• Species: H. sapiens (human)
• Mutation: none
• Entrez Gene: SFRP2 (a.k.a. FRP-2, SARP1, SDF-5)
• Promoter: CMV
• Tags / Fusion Proteins:
  • FLAG (N terminal on backbone)
  • hIgG/Fc (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (not destroyed)
• 3′ cloning site: unknown (not destroyed)
• 5′ sequencing primer: GGA TAG CGG TTT GAC TC
• 3′ sequencing primer: AAA GCC AGA AGA GGG TCT C

Resource Information

• Supplemental Documents:
  • insert sequence information

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Codon Optimized, FLAG-tag is followed by a NotI site and hIgG-tag is preceeded by a XbaI site. The reverse sequencing primer will sequence the CBLN1 insert only and not the hIgG-tag.