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Addgene

pCMV6-XL4 FLAG-CBLN1-Fc
(Plasmid #115765)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115765 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCMV6-XL4
  • Backbone size w/o insert (bp) 111
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CBLN1
  • Species
    H. sapiens (human)
  • Mutation
    none
  • Entrez Gene
    CBLN1
  • Promoter CMV
  • Tags / Fusion Proteins
    • FLAG (N terminal on backbone)
    • hIgG/Fc (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (not destroyed)
  • 3′ cloning site unknown (not destroyed)
  • 5′ sequencing primer GGA TAG CGG TTT GAC TC
  • 3′ sequencing primer AAA GCC AGA AGA GGG TCT C
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Codon Optimized, FLAG-tag is followed by a NotI site and hIgG-tag is preceeded by a XbaI site. The reverse sequencing primer will sequence the CBLN1 insert only and not the hIgG-tag.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV6-XL4 FLAG-CBLN1-Fc was a gift from Davide Comoletti (Addgene plasmid # 115765 ; http://n2t.net/addgene:115765 ; RRID:Addgene_115765)