pCMV6-XL4 FLAG-CBLN1-Fc
(Plasmid
#115765)
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PurposeExpression of the extracellular domain of CBLN1 with N-terminal FLAG tag and C-terminal hIgG tag.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 115765 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV6-XL4
- Backbone size w/o insert (bp) 111
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCBLN1
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SpeciesH. sapiens (human)
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Mutationnone
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Entrez GeneCBLN1
- Promoter CMV
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Tags
/ Fusion Proteins
- FLAG (N terminal on backbone)
- hIgG/Fc (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (not destroyed)
- 3′ cloning site unknown (not destroyed)
- 5′ sequencing primer GGA TAG CGG TTT GAC TC
- 3′ sequencing primer AAA GCC AGA AGA GGG TCT C (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Codon Optimized, FLAG-tag is followed by a NotI site and hIgG-tag is preceeded by a XbaI site. The reverse sequencing primer will sequence the CBLN1 insert only and not the hIgG-tag.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV6-XL4 FLAG-CBLN1-Fc was a gift from Davide Comoletti (Addgene plasmid # 115765 ; http://n2t.net/addgene:115765 ; RRID:Addgene_115765)