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Addgene

p426MET25_sfpHluorin (MRV55)
(Plasmid #115697)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115697 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p246MET25
  • Backbone size w/o insert (bp) 6271
  • Total vector size (bp) 7129
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sfpHluorin
  • Alt name
    superfolder pHluorin
  • Species
    Aequorea victoria
  • Insert Size (bp)
    717
  • Mutation
    for introduced mutations see publication
  • GenBank ID
    M62653.1
  • Promoter MET25

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer GCGTCTGTTAGAAAGGAAGTTTTTCC
  • 3′ sequencing primer ACCTAGACTTCAGGTTGTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p426MET25_sfpHluorin (MRV55) was a gift from Eckhard Boles (Addgene plasmid # 115697 ; http://n2t.net/addgene:115697 ; RRID:Addgene_115697)

  • For your References section:

    A superfolder variant of pH-sensitive pHluorin for in vivo pH measurements in the endoplasmic reticulum. Reifenrath M, Boles E. Sci Rep. 2018 Aug 10;8(1):11985. doi: 10.1038/s41598-018-30367-z. 10.1038/s41598-018-30367-z PubMed 30097598
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