pLV-PGK-SNAP-MDMUT-OCT4
(Plasmid
#115692)
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PurposeConstitutive expression of an OCT4 (Pou5f1) protein tagged with SNAP-tag and a non-functional mitotic degron (MDMUT) for control
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 115692 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLV
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSNAP-MDMUT-OCT4
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SpeciesSynthetic
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Insert Size (bp)1854
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV-PGK-SNAP-MDMUT-OCT4 was a gift from David Suter (Addgene plasmid # 115692 ; http://n2t.net/addgene:115692 ; RRID:Addgene_115692) -
For your References section:
Dynamic regulation of chromatin accessibility by pluripotency transcription factors across the cell cycle. Friman ET, Deluz C, Meireles-Filho AC, Govindan S, Gardeux V, Deplancke B, Suter DM. Elife. 2019 Dec 3;8. pii: 50087. doi: 10.7554/eLife.50087. 10.7554/eLife.50087 PubMed 31794382