pKIOct4-Halo-IRES-Bsd
(Plasmid
#115689)
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PurposeKnock-in of a HaloTag fusion protein into the endogenous OCT4 (Pou5f1) protein
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 115689 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV
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Vector typeMammalian Expression
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameOCT4-Halo-IRES-Bsd
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SpeciesSynthetic
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Insert Size (bp)2023
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/early/2018/04/18/299073 for BioRxiv preprint
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKIOct4-Halo-IRES-Bsd was a gift from David Suter (Addgene plasmid # 115689 ; http://n2t.net/addgene:115689 ; RRID:Addgene_115689) -
For your References section:
Endogenous fluctuations of OCT4 and SOX2 bias pluripotent cell fate decisions. Strebinger D, Deluz C, Friman ET, Govindan S, Alber AB, Suter DM. Mol Syst Biol. 2019 Sep;15(9):e9002. doi: 10.15252/msb.20199002. 10.15252/msb.20199002 PubMed 31556488