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PurposeNanoLuc-Firefly Luciferase fusion with constitutive nuclear expression for calibration of Nanoluc signal to Firefly Luciferase signal. H2B-mCherry marks chromatin with red fluorescence.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 115684 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLV
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Vector typeLentiviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro
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SpeciesSynthetic
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Insert Size (bp)5010
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV-pGK-Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro was a gift from David Suter (Addgene plasmid # 115684 ; http://n2t.net/addgene:115684 ; RRID:Addgene_115684) -
For your References section:
A novel method for quantitative measurements of gene expression in single living cells. Mandic A, Strebinger D, Regali C, Phillips NE, Suter DM. Methods. 2017 May 1;120:65-75. doi: 10.1016/j.ymeth.2017.04.008. Epub 2017 Apr 27. 10.1016/j.ymeth.2017.04.008 PubMed 28456689
