pGM1192
(Plasmid #115683)

• Purpose: E. coli-Streptomyces shuttle vector for gene expression
• Depositing Lab: Günther Muth
• Publication: E.coli-Streptomyces shuttle vectors (unpublished)

Backbone

• Vector backbone: pSG5
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Apramycin, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCherry, aac(3)IV, tsr
• Species: Synthetic; Streptomyces
• Mutation: mCherry codon optimized for Streptomyces
• Promoter: SF14*
• Tag / Fusion Protein:
  • Strep-tagII on mCherry, codon optimized (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: M13 Reverse

Resource Information

• Supplemental Documents:
  • pGM1192.gb
• Addgene Notes:
  • Addgene Diagnostic Digest
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Mobilizable, stable replicating expression vector for Streptomyces, strong constitutive SF14* promoter, codon optimized Strep-tagII-mCherry gene, tsr, aac(3)IV