pTSSX-STC
(Plasmid #115668)

• Purpose: E.coli expression plasmid of small tetraheme cytochrome (STC) from Shewanella oneidensis MR-1 with cleavable Strep tag
• Depositing Lab: David Kramer
• Publication: Huang et al J Am Chem Soc. 2020 Jun 10;142(23):10459-10467. doi: 10.1021/jacs.0c02729. Epub 2020 May 29.

Backbone

• Vector backbone: pTSSX
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: small tetraheme cytochrome c
• Alt name: STC
• Alt name: CctA
• Species: Shewanella oneidensis MR-1
• Insert Size (bp): 393
• GenBank ID: NP_718311
• Promoter: tac
• Tag / Fusion Protein:
  • Signal peptide (aa1-27)-Strep-II tag- Factor Xa cleavage site (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GTTCTGGCAAATATTCTGAAATGAG
• 3′ sequencing primer: CAAATGCCTGAGGTTTCAGC

Resource Information

• Supplemental Documents:
  • pTSSX-STC1.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry