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Addgene

TetON-shYAP
(Plasmid #115667)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115667 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTRIPZ
  • Total vector size (bp) 7000
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    YAP shRNA
  • gRNA/shRNA sequence
    TTATGCCTGTTATGAACCC
  • Species
    H. sapiens (human)
  • Entrez Gene
    YAP1 (a.k.a. COB1, YAP, YAP-1, YAP2, YAP65, YKI)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TetON-shYAP was a gift from Joan Massague (Addgene plasmid # 115667 ; http://n2t.net/addgene:115667 ; RRID:Addgene_115667)

  • For your References section:

    Pericyte-like spreading by disseminated cancer cells activates YAP and MRTF for metastatic colonization. Er EE, Valiente M, Ganesh K, Zou Y, Agrawal S, Hu J, Griscom B, Rosenblum M, Boire A, Brogi E, Giancotti FG, Schachner M, Malladi S, Massague J. Nat Cell Biol. 2018 Aug;20(8):966-978. doi: 10.1038/s41556-018-0138-8. Epub 2018 Jul 23. 10.1038/s41556-018-0138-8 [pii] PubMed 30038252
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