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pKEW106-MbCas12a-58069
(Plasmid #115653)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115653 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CmR/p15A
  • Backbone manufacturer
    N/A
  • Backbone size w/o insert (bp) 2600
  • Total vector size (bp) 6354
  • Vector type
    Bacterial Expression, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MbCas12a
  • Alt name
    MbCpf1
  • Species
    Moraxella bovoculi
  • Insert Size (bp)
    3756
  • GenBank ID
    WP_046700744.1
  • Promoter Ptet

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer tacacctagcttctgggcgagtttacg
  • 3′ sequencing primer gtgagcgaggaagcggaatatatcc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKEW106-MbCas12a-58069 was a gift from Jennifer Doudna (Addgene plasmid # 115653 ; http://n2t.net/addgene:115653 ; RRID:Addgene_115653)

  • For your References section:

    Systematic discovery of natural CRISPR-Cas12a inhibitors. Watters KE, Fellmann C, Bai HB, Ren SM, Doudna JA. Science. 2018 Oct 12;362(6411):236-239. doi: 10.1126/science.aau5138. Epub 2018 Sep 6. 10.1126/science.aau5138 PubMed 30190307
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