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pAav-TP53-T2A-BirA*
(Plasmid #115652)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115652 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAav
  • Backbone manufacturer
    Agilent technologies
  • Backbone size w/o insert (bp) 2907
  • Total vector size (bp) 7416
  • Vector type
    AAV
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    TP53
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    883
  • Mutation
    Homology region 1
  • Entrez Gene
    TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site NdeI (not destroyed)
  • 5′ sequencing primer CACTAGGGGTTCCTGCGGCCGCAAACGCGTCAGCCCTGCACAGACATTTT
  • 3′ sequencing primer TAGGGCGCGATAACTTCGTA
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    TP53
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    838
  • Mutation
    Homology region 2
  • Entrez Gene
    TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer GGGAGGATTGGGAAGACAAT
  • 3′ sequencing primer GGGTTCCTGCGGCCGCTTTTGCTAGCAGATCACGCCACTCCACTCC
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    T2A
  • Species
    Synthetic
  • Insert Size (bp)
    63
  • Tag / Fusion Protein
    • T2A

Cloning Information for Gene/Insert 3

  • Cloning method Unknown
  • 5′ sequencing primer CACTAGGGGTTCCTGCGGCCGCAAACGCGTCAGCCCTGCACAGACATTTT
  • 3′ sequencing primer TAGGGCGCGATAACTTCGTA
    • (Common Sequencing Primers)

Gene/Insert 4

  • Gene/Insert name
    BirA*
  • Species
    E. coli
  • Tag / Fusion Protein
    • BirA*

Cloning Information for Gene/Insert 4

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer ACATATTTGCATGGGGTGTG
  • 3′ sequencing primer TAGGGCGCGATAACTTCGTA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/427807v2 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAav-TP53-T2A-BirA* was a gift from Sven Eyckerman (Addgene plasmid # 115652 ; http://n2t.net/addgene:115652 ; RRID:Addgene_115652)

  • For your References section:

    A Well-Controlled BioID Design for Endogenous Bait Proteins. Vandemoortele G, De Sutter D, Moliere A, Pauwels J, Gevaert K, Eyckerman S. J Proteome Res. 2018 Dec 10. doi: 10.1021/acs.jproteome.8b00367. 10.1021/acs.jproteome.8b00367 PubMed 30525648
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