UR1Ct (265-1040)
(Plasmid
#115647)
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Purposeexpression of truncated Rag1 including the RING, E3 ubiquitin ligase (265-1008) and C-terminal region (384-1040)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 115647 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLEXm
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRag1 (265-1040)
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SpeciesM. musculus (mouse)
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Mutationtruncated Rag1 (aa 265-1040)
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Entrez GeneRag1 (a.k.a. Rag-1)
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Tag
/ Fusion Protein
- 8XHis-MBP-PreScission (N terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
UR1Ct (265-1040) was a gift from Martin Gellert (Addgene plasmid # 115647 ; http://n2t.net/addgene:115647 ; RRID:Addgene_115647) -
For your References section:
Autoinhibition of DNA cleavage mediated by RAG1 and RAG2 is overcome by an epigenetic signal in V(D)J recombination. Grundy GJ, Yang W, Gellert M. Proc Natl Acad Sci U S A. 2010 Dec 28;107(52):22487-92. doi: 10.1073/pnas.1014958107. Epub 2010 Dec 13. 10.1073/pnas.1014958107 PubMed 21149691