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Addgene

coreR1 (384-1008)
(Plasmid #115646)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115646 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLEXm
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rag1 core (384-1008)
  • Species
    M. musculus (mouse)
  • Mutation
    truncated Rag1 (aa 384-1008)
  • Entrez Gene
    Rag1 (a.k.a. Rag-1)
  • Tag / Fusion Protein
    • 8XHis-MBP-PreScission (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    coreR1 (384-1008) was a gift from Martin Gellert (Addgene plasmid # 115646 ; http://n2t.net/addgene:115646 ; RRID:Addgene_115646)

  • For your References section:

    Autoinhibition of DNA cleavage mediated by RAG1 and RAG2 is overcome by an epigenetic signal in V(D)J recombination. Grundy GJ, Yang W, Gellert M. Proc Natl Acad Sci U S A. 2010 Dec 28;107(52):22487-92. doi: 10.1073/pnas.1014958107. Epub 2010 Dec 13. 10.1073/pnas.1014958107 PubMed 21149691
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