eGFP-pk-S65A
(Plasmid
#115619)
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PurposeExpresses human Parkin S65A in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 115619 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C2
- Backbone size w/o insert (bp) 4731
- Total vector size (bp) 6129
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameParkin
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Alt namePARK2
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SpeciesH. sapiens (human)
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Insert Size (bp)1398
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MutationS65A
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GenBank IDNM_004562.2
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Entrez GenePRKN (a.k.a. AR-JP, LPRS2, PARK2, PDJ)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site SalI (unknown if destroyed)
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
eGFP-pk-S65A was a gift from Edward Fon (Addgene plasmid # 115619 ; http://n2t.net/addgene:115619 ; RRID:Addgene_115619) -
For your References section:
Structure-guided mutagenesis reveals a hierarchical mechanism of Parkin activation. Tang MY, Vranas M, Krahn AI, Pundlik S, Trempe JF, Fon EA. Nat Commun. 2017 Mar 9;8:14697. doi: 10.1038/ncomms14697. 10.1038/ncomms14697 PubMed 28276439