eGFP-pk-H302A
(Plasmid #115618)

• Purpose: Expresses human Parkin H302A in mammalian cells
• Depositing Lab: Edward Fon
• Publication: Tang et al Nat Commun. 2017 Mar 9;8:14697. doi: 10.1038/ncomms14697.

Backbone

• Vector backbone: pEGFP-C2
• Backbone size w/o insert (bp): 4731
• Total vector size (bp): 6129
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Parkin
• Alt name: PARK2
• Species: H. sapiens (human)
• Insert Size (bp): 1398
• Mutation: H302A
• GenBank ID: NM_004562.2
• Entrez Gene: PRKN (a.k.a. AR-JP, LPRS2, PARK2, PDJ)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: SalI (unknown if destroyed)
• 5′ sequencing primer: EGFP-C

Resource Information

• Supplemental Documents:
  • eGFP_HsParkin_H302A.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry