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Addgene

pDSG312
(Plasmid #115613)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115613 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSB3K3
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use bacterial strain MG1655 for protein expression.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    regulated adhesin construct
  • GenBank ID
    MH492380

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: Plasmid contains rrnB T1 terminator and T7Te terminator sequences not present in the Genbank sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDSG312 was a gift from Ingmar Riedel-Kruse (Addgene plasmid # 115613 ; http://n2t.net/addgene:115613 ; RRID:Addgene_115613)

  • For your References section:

    A Synthetic Bacterial Cell-Cell Adhesion Toolbox for Programming Multicellular Morphologies and Patterns. Glass DS, Riedel-Kruse IH. Cell. 2018 Jul 26;174(3):649-658.e16. doi: 10.1016/j.cell.2018.06.041. Epub 2018 Jul 19. 10.1016/j.cell.2018.06.041 PubMed 30033369
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