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Addgene

pDSG398
(Plasmid #115604)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115604 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pSB3K3
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use bacterial strain MG1655 for protein expression.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    regulated adhesin construct
  • Mutation
    See depositor comments below
  • GenBank ID
    MH492453

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: Plasmid contains an I578N mutation in the insert compared to the Genbank sequence. This mutation is not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDSG398 was a gift from Ingmar Riedel-Kruse (Addgene plasmid # 115604 ; http://n2t.net/addgene:115604 ; RRID:Addgene_115604)
  • For your References section:

    A Synthetic Bacterial Cell-Cell Adhesion Toolbox for Programming Multicellular Morphologies and Patterns. Glass DS, Riedel-Kruse IH. Cell. 2018 Jul 26;174(3):649-658.e16. doi: 10.1016/j.cell.2018.06.041. Epub 2018 Jul 19. 10.1016/j.cell.2018.06.041 PubMed 30033369