-
Purposesurface-displayed Nb3; medium copy
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 115604 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepSB3K3
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Growth instructionsUse bacterial strain MG1655 for protein expression.
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameregulated adhesin construct
-
MutationSee depositor comments below
-
GenBank IDMH492453
Resource Information
-
Supplemental Documents
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Note: Plasmid contains an I578N mutation in the insert compared to the Genbank sequence. This mutation is not known to affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pDSG398 was a gift from Ingmar Riedel-Kruse (Addgene plasmid # 115604 ; http://n2t.net/addgene:115604 ; RRID:Addgene_115604) -
For your References section:
A Synthetic Bacterial Cell-Cell Adhesion Toolbox for Programming Multicellular Morphologies and Patterns. Glass DS, Riedel-Kruse IH. Cell. 2018 Jul 26;174(3):649-658.e16. doi: 10.1016/j.cell.2018.06.041. Epub 2018 Jul 19. 10.1016/j.cell.2018.06.041 PubMed 30033369