pEBB-XIAPmut
(Plasmid #11559)

• Depositing Lab: Jon Ashwell
• Publication: Yang et al Science 2000 May 5;288(5467):874-7.

Backbone

• Vector backbone: pEBB
• Backbone size w/o insert (bp): 5353
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: XIAPmut
• Alt name: XIAP
• Species: H. sapiens (human)
• Insert Size (bp): 1500
• Mutation: changed his 467 to Alanine
• Entrez Gene: XIAP (a.k.a. API3, BIRC4, IAP-3, ILP1, MIHA, XLP2, hIAP-3, hIAP3)
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: cctattgtgctgatg

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry