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Addgene

Spt2-K166R-GFP
(Plasmid #115573)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115573 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS316
  • Backbone size w/o insert (bp) 4900
  • Total vector size (bp) 6600
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SPT2
  • Alt name
    SIN1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1734
  • Mutation
    Lysine 166 mutated to Arginine
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/345637v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Spt2-K166R-GFP was a gift from Michael Downey (Addgene plasmid # 115573 ; http://n2t.net/addgene:115573 ; RRID:Addgene_115573)

  • For your References section:

    A synthetic non-histone substrate to study substrate targeting by the Gcn5 HAT and sirtuin HDACs. Rossl A, Denoncourt A, Lin MS, Downey M. J Biol Chem. 2019 Apr 19;294(16):6227-6239. doi: 10.1074/jbc.RA118.006051. Epub 2019 Feb 25. 10.1074/jbc.RA118.006051 PubMed 30804216
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