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Addgene

CMV-ShadowY-kCAAX
(Plasmid #115564)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115564 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 3200
  • Total vector size (bp) 4044
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ShadowY with KRas membrane targeting domain
  • Alt name
    ShY_Caax
  • Species
    Synthetic
  • Insert Size (bp)
    810
  • Promoter CMV
  • Tag / Fusion Protein
    • ShadowY (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Age1 (not destroyed)
  • 3′ cloning site BAMHI (not destroyed)
  • 5′ sequencing primer cmv
  • 3′ sequencing primer SV40
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Hideji Murakoshi _shadowY

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

ShadowY developed by Hideji Murakoshi and Akihiro C. E. Shibata
DOI:10.1038/s41598-017-07002-4

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV-ShadowY-kCAAX was a gift from Ryohei Yasuda (Addgene plasmid # 115564 ; http://n2t.net/addgene:115564 ; RRID:Addgene_115564)

  • For your References section:

    PKCalpha integrates spatiotemporally distinct Ca(2+) and autocrine BDNF signaling to facilitate synaptic plasticity. Colgan LA, Hu M, Misler JA, Parra-Bueno P, Moran CM, Leitges M, Yasuda R. Nat Neurosci. 2018 Aug;21(8):1027-1037. doi: 10.1038/s41593-018-0184-3. Epub 2018 Jul 16. 10.1038/s41593-018-0184-3 PubMed 30013171
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