pME-Lifeact-mEos2-NS
(Plasmid
#115549)
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PurposeMultisite gateway vector for 5' tagging with Lifeact-mEos2. Used to make 115563. Parton lab clone IDA
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 115549 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR 221
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLifeact-mEos2
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SpeciesSynthetic
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pME-Lifeact-mEos2-NS was a gift from Rob Parton (Addgene plasmid # 115549 ; http://n2t.net/addgene:115549 ; RRID:Addgene_115549) -
For your References section:
In vivo cell biological screening identifies an endocytic capture mechanism for T-tubule formation. Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim YW, Parton RG. Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w. 10.1038/s41467-020-17486-w PubMed 32709891