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pCDNA3-PAX3-FOXO1/FKHR
(Plasmid #115526)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115526 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pax3-FOXO1/FKHR
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Insert Size (bp)
    2300
  • Entrez Gene
    FOXO1 (a.k.a. FKH1, FKHR, FOXO1A)
  • Entrez Gene
    Pax3 (a.k.a. Pax-3, Sp, Splchl2, splotch)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please see the publication correction regarding this plasmid at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC84722/.
Pax3-FKHR is a mouse-human hybrid composed of murine Pax3 (GenBank accession no. X59358) encoding an amino acid sequence identical to that of human PAX3, followed by human PAX3-FKHR (GenBank accession no. U02368).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3-PAX3-FOXO1/FKHR was a gift from Martine Roussel (Addgene plasmid # 115526 ; http://n2t.net/addgene:115526 ; RRID:Addgene_115526)

  • For your References section:

    The oncogenic potential of the Pax3-FKHR fusion protein requires the Pax3 homeodomain recognition helix but not the Pax3 paired-box DNA binding domain. Lam PY, Sublett JE, Hollenbach AD, Roussel MF. Mol Cell Biol. 1999 Jan;19(1):594-601. PubMed 9858583
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