pUC57kan-T7-gRNA-U6 V2
(Plasmid
#115520)
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PurposeTo construct multiple sgRNA expression vector for CRISPR
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 115520 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC57
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesgRNA scaffolf-U6 promoter
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer M13-fwd (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC57kan-T7-gRNA-U6 V2 was a gift from Xingxu Huang (Addgene plasmid # 115520 ; http://n2t.net/addgene:115520 ; RRID:Addgene_115520)