4xnrUAS:MLS-KillerRed in pTol2pA2-acrys-EGFP-
(Plasmid #115516)

• Purpose: Plasmid driving expression of membrane bound KillerRed fluorophore under UAS response element, with a green lens reporter
• Depositing Lab: Marco Morsch
• Publication: Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi: 10.1016/j.redox.2018.08.011.

Backbone

• Vector backbone: pTol2pA2
• Backbone size w/o insert (bp): 1100
• Total vector size (bp): 7092
• Vector type: Gateway Cloning

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MLS-KillerRed
• Insert Size (bp): 714
• Promoter: UAS
• Tag / Fusion Protein:
  • MLS (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Resource Information

• Supplemental Documents:
  • pDestTol2pAacry-EGFP-4xnrUAS_MLS-KillerRed_pA.gb
• A portion of this plasmid was derived from a plasmid made by: Evrogen
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry