pTol2pA2-mnx1:MLS-KillerRed
(Plasmid
#115515)
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PurposePlasmid driving expression of membrane bound KillerRed fluorophore in zebrafish motor neurons
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 115515 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTol2pA2
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 8126
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Vector typeGateway Cloning
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMLS-KillerRed
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Insert Size (bp)714
- Promoter -3mnx1
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Tag
/ Fusion Protein
- MLS (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byEvrogen
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTol2pA2-mnx1:MLS-KillerRed was a gift from Marco Morsch (Addgene plasmid # 115515 ; http://n2t.net/addgene:115515 ; RRID:Addgene_115515) -
For your References section:
Real-time visualization of oxidative stress-mediated neurodegeneration of individual spinal motor neurons in vivo. Formella I, Svahn AJ, Radford RAW, Don EK, Cole NJ, Hogan A, Lee A, Chung RS, Morsch M. Redox Biol. 2018 Aug 23;19:226-234. doi: 10.1016/j.redox.2018.08.011. 10.1016/j.redox.2018.08.011 PubMed 30193184