pOPS0750
(Plasmid
#115511)
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PurposeReporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing gusA.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 115511 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepOGG026
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namegusA pOGG083, T-pharma pOGG003 assembled in pOGG026
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SpeciesSynthetic
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MutationDomesticated for Golden Gate cloning
- Promoter Constitutive promoter pNeo pOGG001
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Note: Plasmid contains a W83R mutation in GUS. This mutation is not known to affect plasmid function.
Please visit https://www.biorxiv.org/content/early/2018/08/16/392423 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOPS0750 was a gift from Philip Poole (Addgene plasmid # 115511 ; http://n2t.net/addgene:115511 ; RRID:Addgene_115511) -
For your References section:
A Bacterial Expression Vector Archive (BEVA) for Flexible Modular Assembly of Golden Gate-Compatible Vectors. Geddes BA, Mendoza-Suarez MA, Poole PS. Front Microbiol. 2019 Jan 14;9:3345. doi: 10.3389/fmicb.2018.03345. eCollection 2018. 10.3389/fmicb.2018.03345 PubMed 30692983
