pCAGGS-myrGFP
(Plasmid
#115502)
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Purposeexpression of myristoylated, plasma membrane targeted GFP
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 115502 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCAG-GS
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Backbone manufacturerJun-ichi Miyazaki
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemyristoylated GFP
- Promoter chicken beta-actin
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bypCAGGS (Niwa et al. Gene 108, 193-199 (1991)) was obtained from Dr. J. Miyazaki (Osaka University)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAGGS-myrGFP was a gift from Thomas Biederer (Addgene plasmid # 115502 ; http://n2t.net/addgene:115502 ; RRID:Addgene_115502) -
For your References section:
SynCAM 1 participates in axo-dendritic contact assembly and shapes neuronal growth cones. Stagi M, Fogel AI, Biederer T. Proc Natl Acad Sci U S A. 2010 Apr 20;107(16):7568-73. doi: 10.1073/pnas.0911798107. Epub 2010 Apr 5. 10.1073/pnas.0911798107 PubMed 20368431