pcDNA3.1 mSynCAM 4
(Plasmid
#115499)
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Purposeexpression of mouse full-length SynCAM 4
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 115499 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCadm4
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SpeciesM. musculus (mouse)
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Insert Size (bp)1200
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Entrez GeneCadm4 (a.k.a. Igdf4c, Igsf, Igsf4c, Necl, Ts, Tsll2)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1 mSynCAM 4 was a gift from Thomas Biederer (Addgene plasmid # 115499 ; http://n2t.net/addgene:115499 ; RRID:Addgene_115499) -
For your References section:
SynCAMs organize synapses through heterophilic adhesion. Fogel AI, Akins MR, Krupp AJ, Stagi M, Stein V, Biederer T. J Neurosci. 2007 Nov 14;27(46):12516-30. doi: 10.1523/JNEUROSCI.2739-07.2007. 10.1523/JNEUROSCI.2739-07.2007 PubMed 18003830