pCAGGS mSynCAM1
(Plasmid
#115496)
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Purposeexpression of mouse full-length SynCAM 1, CAG promoter suited for neuronal expression
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 115496 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAG-GS
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Backbone manufacturerJun-ichi Miyazaki
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCadm1
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SpeciesM. musculus (mouse)
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Insert Size (bp)1800
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Entrez GeneCadm1 (a.k.a. 2900073G06Rik, 3100001I08Rik, AI987920, Bl2, Igs, Igsf4, Igsf4a, Nec, Necl2, RA1, RA17, RA175, RA175A, RA175B, RA175C, RA175N, ST17, SgI, SgIGSF, Syn, SynCam, Tsl, Tslc1)
- Promoter chicken beta-actin
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bypCAGGS (Niwa et al. Gene 108, 193-199 (1991) was obtained from Dr. J. Miyazaki (Osaka University)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAGGS mSynCAM1 was a gift from Thomas Biederer (Addgene plasmid # 115496 ; http://n2t.net/addgene:115496 ; RRID:Addgene_115496) -
For your References section:
SynCAMs organize synapses through heterophilic adhesion. Fogel AI, Akins MR, Krupp AJ, Stagi M, Stein V, Biederer T. J Neurosci. 2007 Nov 14;27(46):12516-30. doi: 10.1523/JNEUROSCI.2739-07.2007. 10.1523/JNEUROSCI.2739-07.2007 PubMed 18003830